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ZymoBroth™ (ZB) is a specially formulated growth medium used for the preparation of highly competent?E. coli?cells for DNA transformation. When compared to classic SOB growth medium, ZymoBroth™ dramatically increases transformation efficiency, typically on the order of 5 - 100 fold (depending on the?E. coli?strain). As part of our popularMix & Go E. coli?Transformation Kit, ZB enables researchers to generate their own homemade?Mix & Go E. coli?for DNA transformation. ZB medium has been tested on a wide range of?E. coli?strains. Our data indicate that ZB medium stimulates the transformation efficiency of all?E. coli?strains tested, including K12 derivatives (Such as JM109, HB101, etc.) and B strain derivatives (such as BL21, etc.).